Pacific Nanotechnology Inc.

AFM Study of Surface Derivatization for Biosensors

Biotin and Streptavidin Coated Spheres

Quartz spheres are coated with both biotin and streptavidin and AFM images of the spheres were collected. With the AFM it is possible to estimate the percentage of coverage of the sphere.
Left - AFM image of streptavidin coated sphere showing approximately 9% coverage.
Right - AFM image of Biotin coated sphere with approx. 8% coverage.