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Grain Analysis |
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Introduction
The Grain Analysis module is an option for NanoRule+™
image analysis software and is useful for identifying and
analyzing grains. There are several provisions for identifying
grains. Once identified, many parameters such as area,
perimeter, height, and length may be calculated. Several
options are available for reporting the results from the
grain and particle analysis.
Grain boundaries can be defined using threshold levels and
slope in the image. Therefore all grains will be detected at
a threshold level. Two methods, “Threshold Segmentation"
and "Watershed Segmentation", may be used to separate
all regions at the same level.
The five steps required for counting grains in an image are:
Step 1: Prepare Your Image
Step 2: Select Grain Analysis Function
Step 3: Set The Threshold
Step 4: Search and Count Grains
Step 5: Report Grain Analysis Results
Step 1: Prepare Your Image
Before doing Grain Analysis, an image must be leveled to remove any residual background tilt.
Step 2: Select Grain Analysis Function
The Grain Analysis function can be selected from menu Analysis -> Grain Analysis or with the toolbar button.
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When the Grain Analysis dialog box is activated, an image with a highlight is displayed. The highlight is defined by a preset threshold level and detection mode. It may not be optimized for the current image so you may need to adjust this threshold.
Step 3: Set The Threshold
Grain Analysis Dialog Box: In the Grain Analysis Dialog Box,
if the Threshold check box is selected, a defined threshold
level is applied to the image.
Threshold Edit Window: The Threshold Edit Window can
be opened by clicking the "Edit" button in the Threshold
group. An image histogram is then displayed. A
Red/Green line in the histogram window indicates the current
threshold level position. The color bar has two different
palettes, one is the original image palette, and one is a
highlighted palette which means that all data in this range
will be counted when searching for objects.
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