Pacific Nanotechnology Inc.

Guide to AFM standards and references

Bio-Molecules

Often it is necessary to verify SPM image quality on soft samples or samples with weak adhesion to the surface. It is important that this type of reference have a long-life, be stable and be indestructible. Before measuring images of "unknown" biological molecules it is often helpful to practice on this "known" sample.

Company Standard/Reference
NTI-Europe Z = 2nm
Length ~1009nm
Figure 4: Image of DNA that serves as a reference for imaging biological molecules. The diameter of the DNA is 2 nm. Such a sample is used for making sure that the AFM is operating properly and to establish that the operator is proficient. Image size is 1.2 X 1.2 micron
Figure 4: Image of DNA that serves as a reference for imaging biological molecules. The diameter of the DNA is 2 nm. Such a sample is used for making sure that the AFM is operating properly and to establish that the operator is proficient. Image size is 1.2 X 1.2 micron