SPM height calibration can be done on nano-spheres of uniform size, it also verifies SPM performance on samples with weak adhesion to the surface. Often it is helpful to include nanoparticles with a sample to establish the sizes of features in the imager relative to the nanoparticles.
All colors of q-dots. Quantum dots of any size come in great variety of kits.
Figure 3: This AFM image is of 102 nm diameter polymer nanospheres. Software can be used for counting the particles and for measuring a size distribution of the partcles.
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